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Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 522-527 doi: 10.1007/s11684-017-0526-7

Abstract: Detecting circulating tumor cell (CTC) or circulating tumor DNA (ctDNA) in plasma or serum could serve

Keywords： liquid biopsy     circulating tumor cells     cell-free ctDNA     exosomes     precision medicine    

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Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 224-229 doi: 10.1007/s11705-008-0029-9

Abstract: The cell-free protein synthesis system provides a new strategy of protein expression with advantagessystem are the key factors for the successful construction of cell-free protein expression system.In this work, the cell extract was prepared from RNase I- defective strain A19.The cell growth phase, the pressure for cell disruption and the storage condition of cell extracts wereUnder the optimized conditions, the green fluorescent protein (GFP) reporter gene was expressed in the cell-free

Keywords： cell-free     regeneration     extract     information     successful construction    

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Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 58-65 doi: 10.1007/s11705-017-1610-x

Abstract: The advent of synthetic biology has ushered in new applications of cell-free transcription-translationThese cell-free systems are reconstituted using cellular proteins, and are amenable to modular controlHere, we discuss the historical advancement of cell-free systems, as well as their new applications inFinally, we present our vision on the future direction of cell-free synthetic biology.

Keywords： cell-free system     application    

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Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 526-532 doi: 10.1007/s11783-010-0261-7

Abstract: cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free

Keywords： microcystin-LR(MC-LR)     biodegradation     Sphingopyxis sp     USTB-05     cell-free extract    

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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering Article

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Engineering 2018, Volume 4, Issue 6,   Pages 888-893 doi: 10.1016/j.eng.2018.09.007

Abstract: The enzymatic activities of the R61A, L88F, and I179F mutants, which were obtained with a rapid cell-free

Keywords： Polyesterase     PET degradation     Cell-free protein synthesis     Polyester     PETase    

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MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things Article

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Engineering 2022, Volume 8, Issue 1,   Pages 96-107 doi: 10.1016/j.eng.2021.11.002

Abstract: Thus, we can build a non-terrestrial network (NTN) using a cell-free architecture.

Keywords： Cell-free Mobile edge computing     Non-terrestrial networks     Sixth-generation     Wide-area time-sensitive    

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An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis

M. H. NGUYEN-THOI,L. Le-ANH,V. Ho-HUU,H. Dang-TRUNG,T. NGUYEN-THOI

Frontiers of Structural and Civil Engineering 2015, Volume 9, Issue 4,   Pages 341-358 doi: 10.1007/s11709-015-0302-1

Abstract: A cell-based smoothed discrete shear gap method (CS-FEM-DSG3) was recently proposed and proven to berobust for free vibration analyses of Reissner-Mindlin shell.The method improves significantly the accuracy of the solution due to softening effect of the cell-basedThe paper hence tries to extend the CS-FEM-DSG3 for free vibration analysis of cracked Reissner-MindlinThe accuracy and reliability of the novel XCS-FEM-DSG3 for free vibration analysis of cracked Reissner-Mindlin

Keywords： cracked Reissner-Mindlin shell     free vibration analysis     cell-based smoothed discrete shear gap method(CS-FEM-DSG3)     extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3)     smoothed finite element    

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NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis

Gang WANG

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 1-7 doi: 10.1007/s11684-009-0018-5

Abstract: Reactive oxygen species (ROS) are small molecule metabolites of oxygen that are prone to participate in redox reactions their high reactivity. Intracellular ROS could be generated in reduced nicotinamide-adenine dinucleotidephosphate (NADPH) oxidase-dependent and/or NADPH oxidase-independent manners. Physiologically, ROS are involved in many signaling cascades that contribute to normal processes. One classical example is that ROS derived from the NADPH oxidase and released in neurotrophils are able to digest invading bacteria. Excessive ROS, however, contribute to pathogenesis of various human diseases including cancer, aging, dimentia and hypertension. As signaling messengers, ROS are able to oxidize many targets such as DNA, proteins and lipids, which may be linked with tumor growth, invasion or metastasis. The present review summarizes recent advances in our comprehensive understanding of ROS-linked signaling pathways in regulation of tumor growth, invasion and metastasis, and focuses on the role of the NADPH oxidase-derived ROS in cancer pathogenesis.

Keywords： free radicals     tumor     phox     cell proliferation     cancer therapy    

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Comparison of reference values for immune recovery between event-free patients receiving haploidentical

Xuying Pei, Xiangyu Zhao, Yu Wang, Lanping Xu, Xiaohui Zhang, Kaiyan Liu, Yingjun Chang, Xiaojun Huang

Frontiers of Medicine 2018, Volume 12, Issue 2,   Pages 153-163 doi: 10.1007/s11684-017-0548-1

Abstract:

To establish optimal reference values for recovered immune cell subsets, we prospectively investigatedpost-transplant immune reconstitution (IR) in 144 patients who received allogeneic stem cell transplantationthat data from this recipient cohort should be used as reference values for post-transplant immune cell

Keywords： immune reconstitution     hematopoietic stem cell transplantation     event-free patients     reference range    

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The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,

Xinxin LI,Huijuan WANG,Guanghua SU,Zhuying WEI,Chunling BAI,Wuni-MENGHE,Yanhui HOU,Changqing YU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 191-200 doi: 10.15302/J-FASE-2014029

Abstract: Since the birth of the first cloned sheep, somatic cell nuclear transfer technology has been successfullyIn the present study, the cloned Dorper sheep equipped with GPS location devices were free-grazed inThe main findings of this research were as follows. (1) Under free-grazing conditions, the cloned sheepsheep. (3) The cloned sheep exhibited free-grazing patterns and social behavior as other sheep. (4)The Dorper sheep readily adapted to the free-grazing conditions on the Mongolian plateau grassland, which

Keywords： somatic cell nuclear transfer     free-grazing synchronization     Dorper sheep     cloned animal ecology    

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Minimal residual disease in solid tumors: an overview

Frontiers of Medicine 2023, Volume 17, Issue 4,   Pages 649-674 doi: 10.1007/s11684-023-1018-6

Abstract: Minimal residual disease (MRD) is termed as the small numbers of remnant tumor cells in a subset of patients with tumors. Liquid biopsy is increasingly used for the detection of MRD, illustrating the potential of MRD detection to provide more accurate management for cancer patients. As new techniques and algorithms have enhanced the performance of MRD detection, the approach is becoming more widely and routinely used to predict the prognosis and monitor the relapse of cancer patients. In fact, MRD detection has been shown to achieve better performance than imaging methods. On this basis, rigorous investigation of MRD detection as an integral method for guiding clinical treatment has made important advances. This review summarizes the development of MRD biomarkers, techniques, and strategies for the detection of cancer, and emphasizes the application of MRD detection in solid tumors, particularly for the guidance of clinical treatment.

Keywords： MRD     solid tumor     CTC     ctDNA    

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Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

Frontiers in Energy 2018, Volume 12, Issue 2,   Pages 233-238 doi: 10.1007/s11708-018-0529-3

Abstract: the anodic response to glucose detection leads to a higher overall performance of enzymatic biofuel cellThus, here we first synthesized N-graphene via a catalyst-free single-step thermal process, and made

Keywords： enzymatic fuel cell     nitrogen-doped graphene     reduced graphene oxide     catalyst-free synthesis    

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Efficient photoelectrochemical oxidation of rhodamine B on metal electrodes without photocatalyst or supporting electrolyte

Xuejiao Wang, Xiang Feng, Jing Shang

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 6, doi: 10.1007/s11783-018-1061-8

Abstract:

•The efficient PEC degradation of RhB is realized using no photocatalyst.

•The efficient PEC degradation of RhB features the low salinity.

•The PEC degradation of RhB takes place on the anode and cathode simultaneously.

Keywords： Energy relay structure     Energy saving     Photocatalyst-free and low-salinity degradation     Photoelectrochemicalcell    

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Recent advances in cycloaddition of CO with epoxides: halogen-free catalysis and mechanistic insights

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 12,   Pages 1879-1894 doi: 10.1007/s11705-023-2354-4

Abstract: To address these concerns, the halogen-free cycloaddition of CO2 with epoxides has alwaysIn this review, we systematically discussed the halogen-free catalysis for cycloaddition of CO2freeSpecially, metal oxides as the potential halogen-free catalysts are highlighted due to their flexible

Keywords： carbon dioxide     halogen-free catalysis     cyclic carbonate     mechanistic insight    

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Hollow carbon spheres and their noble metal-free hybrids in catalysis

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 6,   Pages 1380-1407 doi: 10.1007/s11705-021-2097-z

Abstract: Herein, we overview hollow carbon sphere-based materials and their noble metal-free hybrids in catalysiscontrolling pore structure and surface morphology, and then heterogeneous doping as well as their metal-free

Keywords： hollow carbon spheres     functionalization     noble metal-free     catalysis    

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Title Author Date Type Operation

Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Journal Article

Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Journal Article

Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Journal Article

Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Journal Article

Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Journal Article

MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Journal Article

An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis

M. H. NGUYEN-THOI,L. Le-ANH,V. Ho-HUU,H. Dang-TRUNG,T. NGUYEN-THOI

Journal Article

NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis

Gang WANG

Journal Article

Comparison of reference values for immune recovery between event-free patients receiving haploidentical

Xuying Pei, Xiangyu Zhao, Yu Wang, Lanping Xu, Xiaohui Zhang, Kaiyan Liu, Yingjun Chang, Xiaojun Huang

Journal Article

The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,

Xinxin LI,Huijuan WANG,Guanghua SU,Zhuying WEI,Chunling BAI,Wuni-MENGHE,Yanhui HOU,Changqing YU,Shorgan BOU,Guangpeng LI

Journal Article

Minimal residual disease in solid tumors: an overview

Journal Article

Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

Journal Article

Efficient photoelectrochemical oxidation of rhodamine B on metal electrodes without photocatalyst or supporting electrolyte

Xuejiao Wang, Xiang Feng, Jing Shang

Journal Article

Recent advances in cycloaddition of CO with epoxides: halogen-free catalysis and mechanistic insights

Journal Article

Hollow carbon spheres and their noble metal-free hybrids in catalysis

Journal Article